Porcine Cortisol ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-OSEL-P0002-24, E-OSEL-P0002-48, E-OSEL-P0002-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: Cortisol

UNIProt ID: ???

Target Synonyms: 11β; 17α; 21-Trihydroxypregn-4-ene-3; 20-dione;

Research Areas: Cell biology, Metabolism

Target Species: Porcine

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Porcine Cortisol concentrations in serum, plasma.

Tested Sample Types: Serum, Plasma

Type: Competitive

Assay Time: 1.5h

Detection Type: Colormetric

Sensitivity: 1.41 ng/mL

Detection Range: 3.13-200 ng/mL

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Porcine Cortisol are added to the micro ELISA plate wells. Porcine Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Porcine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Porcine Cortisol in samples. No significant cross-reactivity or interference between Porcine Cortisol and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃, 6 months

Shipping: ice bag