Human IRS1 (Insulin Receptor Substrate 1) ELISA Kit

Sizes: 24T, 48T, 96T

Catalogue Numbers: E-EL-H5554-24, E-EL-H5554-48, E-EL-H5554-96

Citations, Manuals and MSDS Available upon request.

Abbreviation: IRS1

UNIProt ID: P35568

Research Areas: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction, Tags and Cell Markers

Target Species: Human

Application: ELISA

Usage: This ELISA kit applies to the in vitro quantitative determination of Human IRS1 concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, Plasma and other biological fluids

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.1 ng/mL

Detection Range: 0.16-10 ng/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IRS1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IRS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IRS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IRS1. You can calculate the concentration of Human IRS1 in the samples by comparing the OD of the samples to the standard curve.

Cross Reactivity: This kit recognizes Human IRS1 in samples. No significant cross-reactivity or interference between Human IRS1 and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 10%.

Storage: 2-8℃/-20℃, 12 months

Shipping: ice bag