CA II Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90158-50, BS90158-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P00918(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IHC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme. The human CA4 gene, which maps to chromosome 17q23, encodes CA IV, a membrane-anchored isozyme that is expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression. CA IX is specifically expressed in clear-cell renal carcinomas, whereas CA XII is highly expressed in normal tissues, such as kidney, colon and pancreas. Human CA XIV is also expressed in normal tissues, such as brain, but differs from CA XII in its expression pattern.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: CA II polyclonal antibody detects endogenous levels of CA II protein.

Bioworld Molecular Weight: 29 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Carbonic anhydrase 2 on different cell lysates using anti-Carbonic anhydrase 2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293
Lane 2: THP-1
Lane 3: HL-60, ICC staining Carbonic anhydrase 2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CAC, CA-II, CA2, can, CAN_ECOLI, Carbonate dehydratase 2, Carbonic anhydrase 2,

Immunogen: Recombinant protein with human Carbonic anhydrase 2 aa 50-220.

Conjugate: Unconjugated

Modification: Unmodified