MMP1 (Cleaved-Ile271) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES20022-50, ES20022-100

Citations, Manuals and MSDS Available upon request.

Background: catalytic activity:Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1 (I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue., cofactor:Binds 2 zinc ions per subunit., cofactor:Binds 4 calcium ions per subunit., domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme., domain:There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases)., enzyme regulation:Can be activated without removal of the activation peptide., function:Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity., online information:Collagenase entry, PTM:Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase., similarity:Belongs to the peptidase M10A family., similarity:Contains 4 hemopexin-like domains., subunit:Interacts with HIV-1 Tat.,

Alternate Name: Interstitial collagenase (EC 3.4.24.7; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1) [Cleaved into: 22 kDa interstitial collagenase; 27 kDa interstitial collagenase]

Source: Rabbit

Applications: WB; ELISA; IHC

Dilution: WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000

Reactivity: Human; Rat; Mouse;

Immunogen: Synthesized peptide derived from human MMP1 (Cleaved-Ile271)

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 22 53kD

Human Gene ID: 4312

Human SWISS Prot NO: P03956

Subcellular Location: Secreted, extracellular space, extracellular matrix.

Research Use Only