Phospho-Rb (Ser780) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16380-50, BZ16380-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P06400

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 106 kDa; Observed MW: 106 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-Rb in various lysates using Phospho-Rb antibody., Western blot analysis of Phospho-Rb in KB lysates using Phospho-Rb antibody., Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Retinoblastoma antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right., Western blot analysis of Phospho-Rb in K562 lysates treated with serum 10% using Phospho-Rb antibody. The Lane on the right is blocked with the Phospho- peptide.

Alternative Name: RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110

Immunogen: The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780. AA range:751-800

Conjugate: Unconjugated

Modification: Phosphorylated