BPI Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90144-50, BS90144-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17213(Human)

Host: Rabbit

Reactivity: Human, zebrafish

Applications: WB, FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100

Background: The bactericidal permeability increasing protein (BPI) is an antibacterial and endotoxin-neutralizing molecule that is abundant in the granules of polymorphonuclear leukocytes (neutrophil granules). The 31.5-kb-long human BPI gene maps to chromosome 20q11.23-q12, contains 15 exons, and encodes a 456 amino acid protein. Epithelial cells which line mucosal surfaces are the first line of defense against bacterial invasion and infection. BPI localizes to the cell surface of epithelial cells and blocks endotoxin-mediated signaling, thereby protecting mucosal surfaces against gram-negative bacteria and their endotoxin. BPI, lipopolysaccharide binding protein (LBP), phospholipid transfer protein (PLTP), and cholesteryl ester transfer protein (CETP) constitutes a family of functionally related proteins.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: BPI polyclonal antibody detects endogenous levels of BPI protein.

Bioworld Molecular Weight: 57 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of BPI on THP-1 cells lysates using anti-BPI antibody at 1/1,000 dilution., Flow cytometric analysis of Jurkat cells with BPI antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Alternative Name: BPI, Bactericidal permeability-increasing protein, bactericidal/permeability-increasing protein, BPI, BPI fold containing family D, member 1, BPIFD1, CAP 57, rBPI, recombinant BPI holoprotein, rBPI,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified