Mad2L1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90819-50, BS90819-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13257(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC

All Applications: WB:1:500-1:2,000
ICC:1:100-1:500

Background: Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T47D, was shown to complete mitosis in the presence of nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during prometaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with insulin receptor (IR), but not IGFIR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and if the process is not complete, MAD2B delays the onset of anaphase.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Mad2L1 polyclonal antibody detects endogenous levels of Mad2L1 protein.

Bioworld Molecular Weight: 23.5 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Mad2L1 on different cell lysates using anti-Mad2L1 antibody at 1/500 dilution.
Positive control:
Lane 1: K562
Lane 2: 293T
Lane 3: SH-SY5Y, ICC staining Mad2L1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HsMAD2, MAD2-like protein 1, MAD2L1, MAD2, Caught by MAD2 protein, CMT2, dJ261G23.1, KIAA0110, MAD2L1 binding protein, MAD2L1 binding protein isoform 2, MAD2L1-binding protein, Mad2l1bp, MD2BP, MGC11282, OTTHUMP00000016496, RP1 261G23.6,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified